Ethidium bromide solution msds sheet

2. To one tube, add 10 μL of Solution 1 and then 10 μL of Solution 2. To the other tube add 20 μL of distilled water. 3. Briefly vortex and microcentrifuge both tubes, and let sit at room temperature for 5 minutes. 4. Electrophorese both samples on a 1% agarose gel stained with ethidium bromide and view with UV light.

Ethidium bromide can be added to YPD media and used as an inhibitor for cell growth. [24] Handling and disposal. Ethidium bromide is not regulated as hazardous waste at low concentrations, [25] but is treated as hazardous waste by many organizations. Material should be handled according to the material safety data sheet (MSDS). ALPHA CHEMIKA from Mumbai, India is a manufacturer, supplier and exporter of Mercuric Chloride, Lanthanum Acetate at an affordable price.

This chemical inventory is OSHA's premier one-stop shop for occupational chemical information. It compiles information from several government agencies and organizations. Information available on the pages includes: Chemical identification and physical properties Exposure limits Sampling information ... Fact Sheet EH&S Facts Ethidium Bromide (CAS# 1239-45-8) 11/2010 What is Ethidium Bromide? Ethidium bromide (EtBr) is commonly used as a non-radioactive DNA stain to identify and visualize nucleic acid bands in electrophoresis and perform other methods of nucleic acid separation. Solutions of EtBr fluoresce readily with a reddish-brown color A 2 % solution of agarose is prepared by boiling 5 g of agarose in 250 ml of 1x TBE until the solution becomes completely dissolved. By stirring the solution cool it down to < 60°C and add 4µl of ethidium bromide. Subsequently pour the agarose solution – devoid of bubbles - into a prepared and sealed gel tray. Ethidium bromide is a sensitive fluorescent stain for visualizing DNA in agarose and polyacrylamide gels. It is excited with a standard 302 nm UV transilluminator and emits a red-orange signal that can be photographed with Polaroid film or with a CCD-based gel documentation system. Ethidium Bromide Solution

Ethidium Bromide. Storage and Handling • GelStar™ Stain is supplied as a 10,000X concentrated stock solution in DMSO. Store desiccated at -20°C protected from light. When stored per directions the stock solution is stable for 12 months from the date of receipt. • Allow time for the stock solution to thaw totally. benzene, chloroform, dimethyl formamide, ethidium bromide, mercury, methylene chloride, and zinc chloride. Material Safety Data Sheets (MSDSs) provide information regarding reproductive hazards of chemicals. Additional information regarding chemical as well as other reproductive hazards (e.g., viruses, bacteria, ionizing radiation, etc.) is A 10 mg/mL Ready-To-Use solution of EtBr in specially filtered, ... Material Safety Data Sheet ... Ethidium bromide intercalates double-stranded DNA and RNA and acts ...